Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells

Exing Wang, Janice Pennington, James R. Goldenring, Walter Hunziker and Kenneth W. Dunn**




      Wangetal2.pdf Journal article in pdf format.

     Movie 1A.  Polarized MDCK cells incubating in apical Alexa488-Tf and basolateral Alexa568-Tf.
     Movie 1B.  Polarized MDCK cells incubating in apical Alexa488-Tf and basolateral Alexa568-Tf after treatment with BFA.

     Movie 3A.  Polarized MDCK cells incubating in basolateral Oregon Green-IgA and TxR-Tf.
     Movie 3B.  Polarized MDCK cells incubating in basolateral Oregon Green-IgA and TxR-Tf after treatment with BFA

     Movie 5A.  Polarized MDCK cells stably expressing GFP-Rab25.
     Movie 5B.  Polarized MDCK cells stably expressing GFP-Rab25 treated with BFA.

     Movie 6A.  Polarized MDCK cells incubating in basolateral FR-Tf.
     Movie 6B.  Polarized MDCK cells incubating in basolateral FR-Tf following treatment with BFA.
     Movie 7L.  Polarized MDCK cells incubating in basolateral Alexa488-Tf and diI-LDL following treatment with BFA.
 

**Corresponding author can be reached at: kwdunn@iupui.edu  or

Kenneth Dunn, Ph.D.
Division of Nephrology
Fesler Hall Rm. 115
1120 South Dr.
Indianapolis, IN 46202
(317)278-0436