Three dimensional imaging of embryonic mouse kidney by two-photon microscopy

Carrie Phillips, Lois Arend, Adele Filson, Doug Kojetin, Jeff Clendenon, Shiaofen Fang and Kenneth Dunn

American Journal of Pathology, Vol. 158, No. 1 (January, 2001), pp. 49-55

For best results, completely download QuickTime movies first, and then use progress bar in QuickTime Player to manipulate animation.  IVIE animations are created at 6 frames per second, and manipulating the QuickTime animation via the progress bar approximates the interactive experience of its “real-time” rendering. For additional information, please contact Carrie Phillips cphilli3@iupui.edu

phillipsetal.pdf  - Journal article available in pdf format.


Figure 1 A – Embryonic day 11.5 kidney labeled with peanut agglutinin
Rotating projection   (1.5M)


Figure 1 B – Embryonic day 13.5 kidney labeled with peanut agglutinin
Rotating projection   (4.4M)


Figure 1 C – Embryonic day 13.5 kidney labeled with soybean agglutinin
Rotating projection   (5.3M)

 
 


Figure 2 A – Embryonic day 17.5 kidney labeled with peanut agglutinin
Rotating projection   (4.3M)

IVIE rendering   (8.6M)


Figure 2 B – Embryonic day 17.5 kidney labeled with peanut agglutinin
Rotating projection   (3.7M)

IVIE rendering   (7.7M)


Figure 2 C – Embryonic day 17.5 kidney labeled with phalloidin
Rotating projection   (6.9M)

 
 


Figure 3 A – Newborn wild-type newborn mouse kidney labeled with Lens culinaris lectin
Rotating projection   (3.8M)

IVIE rendering   (6.2M)


Figure 3 B – Newborn wild-type mouse kidney labeled with peanut agglutinin
Rotating projection   (4.5M)

IVIE rendering   (6.7M)


Figure 3 C – Newborn inv/inv mouse kidney labeled with peanut agglutinin
Rotating projection   (3.3M)

IVIE rendering (5.4M)

 


Figure 6 A – Embryonic day 17.5 kidney labeled with peanut agglutinin (red) and Lens culinaris lectin (green)
Rotating projection   (4.1M)


Figure 6 B – Newborn inv/inv mouse kidney labeled with peanut agglutinin (red) and Tamm-Horsfall protein (green)
Rotating projection   (2.1M)