2003 Indiana University Division of Nephrology Microscopy Workshop
September 15-20, 2003
OPTICAL MICROSCOPY IN RENAL RESEARCH
As part of the educational component of the recent NIH George M. O’Brien Center award, the Indiana Center for Biological Microscopy presented a 6 day workshop entitled, "Optical Microscopy in Renal Research" on September 15-20, 2003. The workshop provided renal investigators with training and hands-on experience with various modes of optical microscopy.
LECTURES provided participants with the background necessary to assemble microscope systems and to design and conduct studies. Topics will included:
Microscopy: Understanding fundamental principles
and what’s new
Selecting a fluorescence microscopy system based
on your specific needs
Strategies to ensure optimal image collection
The spectrum of vital microscopy A,
B
Introduction to Volume Rendering
Strategies of fluorescence labeling and sample preparation to maximize interpretation
Obtaining and optimizing a system of your own
LABORATORY:Exercises emphasized hands-on experience with multiple instruments for digital image collection and analysis. Participants conducted studies with the following equipment:
Bio-Rad MRC-1024
confocal/multiphoton microscope
Zeiss
510-NLO-META confocal/multiphoton microscope
Zeiss 510-UV
confocal microscope
Perkin-Elmer
Ultraview High-speed confocal microscope
Applied
Precision Deltavision image deconvolution microscope system
Pentium-based
image processing workstations with Metamorph and Voxx rendering software
Laboratory exercises included:
Alignment and
adjustment of the light microscope for transillumination and epifluorescence
Collection of
digital images: balancing the tradeoffs according to experimental needs
Evaluating and
optimizing a fluorescence microscope system: measuring efficiency and noise
characteristics
Collection and
analysis of 3-dimensional images: digital deconvolution, confocal microscopy
and multi-photon microscopy
Imaging dynamics
of living cells and tissues in 2 and 3 dimensions
Intravital
microscopy: multiphoton microscopy of the kidney of a living rat
Quantitative
microscopy: ratiometric ion studies and evaluations of co-localization
Participants were encouraged to bring experimental materials to the workshop as center equipment and faculty will be available during evening hours for investigators to conduct pilot studies.
2003 COURSE FACULTY & LECTURERS:
Simon Atkinson -
Indiana University School of Medicine
Robert Bacallao
- Indiana University School of Medicine
Dennis Brown -
Harvard Medical School
Kenneth Dunn -
Indiana University School of Medicine
Marshall
Montrose - Indiana University School of Medicine
Carrie Phillips -
Indiana University School of Medicine
Kenneth Spring -
NIH, National Heart, Lung and Blood Institute
George Tanner -
Indiana University School of Medicine
Sam Wells -
Vanderbilt Medical Center
COST:
Registration for the Obrien Course was $625.00
Six nights single occupancy at the University Place
Hotel on Campus was $678.00 plus tax (optional)
Six nights double occupancy was $384.00 plus tax
(optional)