Publications

Indiana Center for Biological Microscopy

In addition to providing microscopy support for numerous NIH-supported investigators, the Center is also actively involved in research into biological imaging, resulting in the development and dissemination of new methods of microscopy and digital image analysis software. The products of these activities are disseminated through a program of education, including seminars, courses and individual training. In 2002 the ICBM was designated as an NIDDK George O’Brien Center for Advanced Microscopic Analysis, providing $5.2 million dollars for development and dissemination of microscopy methods for renal research. Publications resulting from ICBM imaging research, and utilizing ICBM imaging facilities are listed below.

Ashworth SL, Sandoval RM, Tanner GA, Molitoris BA. Two-photon microscopy: visualization of kidney dynamics. Kidney Int. 2007 Aug;72(4):416-21. Review.

Molitoris BA, Sandoval RM. Quantifying dynamic kidney processes utilizing multi-photon microscopy. Contrib Nephrol. 2007;156:227-35. Review.

Yu, W. 2006. Quantitative microscopic approaches for studying kidney functions. Nephron Physiol. 103:p63-70.

Wang, E., Babbey, C.M., and Dunn, K.W. 2005. Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems. Journal of Microscopy. 218:148-149.

Rubart M. Two-photon microscopy of cells and tissue. Circ Res. 2004 Dec 10; 95(12): 1154-66.

Molitoris, B and Sandoval R. Mulit-Photon Microscopy of Dynamic Renal Processes. (Invited review submitted to the American Journal of Physiology: Renal Physiology, Dec 2004.)

Dunn, K.W., Sandoval, R. and B. Molitoris. 2003. Intravital imaging of the kidney using multi-parameter, multi-photon microscopy. Nephron, 94:e7-e11.

Bacallao RL, Yu W, Dunn KW, Phillips CL: Novel light microscopy imaging techniques in nephrology. Current Opinion in Nephrology and Hypertension, 12: 455-481 (2003).

Dunn, K., R. Sandoval, K. Kelly, P. Dagher, G. Tanner, S. Atkinson, R. Bacallao and B. Molitoris. 2002. Functional studies of the kidney of living animals using multicolor 2-photon microscopy. Am. J. Physiol. (Cell). 282:C905-C916.

J.L. Clendenon, C.L. Phillips, R.M. Sandoval, S. Fang, K.W. Dunn.Voxx: a PC-based, near real-time volume rendering system for biological microscopy. American Journal of Physiology - Cell Physiology, 282, 1 (January, 2002), pp. C213-C218.

Phillips, C.L., L.J. Arend, A.J. Filson, D.J. Kojetin, J.L. Clendenon, S. Fang, and K.W. Dunn. 2001. Three-dimensional imaging of embryonic mouse kidney by two-photon microscopy. Am J Pathol. 158:49-55.

Dunn, K.W. and E. Wang. 2000. Optical aberrations and objective choice in multi-color confocal microscopy. Biotechniques. 28: 542-550.

Fang, S., Y. Dai, F. Myers, M. Tuceryan and K. Dunn. 2000. Three-dimensional data exploration by interactive volume visualization. Scanning. 22:218-226.

Dunn, K. and F. Maxfield. 1998. Ratio imaging instrumentation. Methods in Cell Biology. 56: 217-236.

Dunn, K. and R. Bacallao. 1998. Optical methods in renal research. Seminars in Neph. 18:122-137.

Publications Utilizing the Imaging Facility

Peer reviewed publications resulting from Indiana Center for Biological Microscopy Resources are listed by year below. Papers in which images acquired either contributed to the body of the work or appeared as part of the published paper are shown.

Basu S, Ray NT, Atkinson SJ, Broxmeyer HE. Protein phosphatase 2A plays an important role in stromal cell-derived factor-1/CXC chemokine ligand 12-mediated migration and adhesion of CD34+ cells. J Immunol. 2007 Sep 1;179(5):3075-85.

Brutkiewicz RR, Willard CA, Gillett-Heacock KK, Pawlak MR, Bailey JC, Khan MA, Nagala M, Du W, Gervay-Hague J, Renukaradhya GJ. Protein kinase C delta is a critical regulator of CD1d-mediated antigen presentation. Eur J Immunol. 2007 Sep;37(9):2390-5.

Chen J, He R, Minshall RD, Dinauer MC, Ye RD. Characterization of a mutation in the Phox homology domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production. J Biol Chem. 2007 Oct 12;282(41):30273-84.

Chen NX, O'Neill KD, Chen X, Duan D, Wang E, Sturek MS, Edwards JM, Moe SM. Fetuin-A uptake in bovine vascular smooth muscle cells is calcium dependent and mediated by annexins. Am J Physiol Renal Physiol. 2007 Feb;292(2):F599-606.

Dai, W, LJ Field, M Rubart, S Reuter, SL Hale, R Zweigerdt, RE Graichen, GL Kay, AJ Jyrala, A Colman, BP Davidson, M Pera and RA Kloner. Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts. J Mol Cell Card, 43: 504-516.

Dominguez JH, Mehta JL, Li D, Wu P, Kelly KJ, Packer CS, Temm CJ, Goss E, Cheng L, Zhang S, Patterson CE, Hawes JW, Peterson RG. Anti-LOX-1 therapy in rats with diabetes and dyslipidemia: Ablation of renal vascular and epithelial manifestations. Am J Physiol Renal Physiol. 2007 Nov 7;

Dominguez J, Wu P, Packer CS, Temm C, Kelly KJ. Lipotoxic and inflammatory phenotypes in rats with uncontrolled metabolic syndrome and nephropathy. Am J Physiol Renal Physiol. 2007 Sep;293(3):F670-9.

El-Achkar TM, Plotkin Z, Marcic B, Dagher PC. Sepsis induces an increase in thick ascending limb Cox-2 that is TLR4 dependent. Am J Physiol Renal Physiol. 2007 Oct;293(4):F1187-96.

Gopalakrishnan S, Hallett MA, Atkinson SJ, Marrs JA. aPKC-PAR complex dysfunction and tight junction disassembly in renal epithelial cells during ATP depletion. Am J Physiol Cell Physiol. 2007 Mar;292(3):C1094-102.

Gupta A, Rhodes GJ, Berg DT, Gerlitz B, Molitoris BA, Grinnell BW. Activated protein C ameliorates LPS-induced acute kidney injury and downregulates renal INOS and angiotensin 2. Am J Physiol Renal Physiol. 2007 Jul;293(1):F245-54.

Hörbelt M, Lee SY, Mang HE, Knipe NL, Sado Y, Kribben A, Sutton TA. Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury. Am J Physiol Renal Physiol. 2007 Sep;293(3):F688-95.

Hörbelt M, Wotzlaw C, Sutton TA, Molitoris BA, Philipp T, Kribben A, Fandrey J, Pietruck F. Organic cation transport in the rat kidney in vivo visualized by time-resolved two-photon microscopy. Kidney Int. 2007 Aug;72(4):422-9.

Horbelt, M., Young, S., Mang, H.E., Knipe, N.L., Sado, Y., Kribben, A., and Sutton, T.A.. Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury. Am. J. Physiol. Renal Physiol, 293: F688-F695, 2007

Ito S, Mantel CR, Han MK, Basu S, Fukuda S, Cooper S, Broxmeyer HE. Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells. Blood. 2007 Mar 1;109(5):1923-30.

Kher R, Bacallao RL. Imaging gene expression. Nephron Exp Nephrol. 2006;103(2):e75-80. Review.

Kwon, O., S.M. Hong, and K. Blouch. 2007. Alteration in Renal Organic Anion Transporter 1 After Ischemia/Reperfusion in Cadaveric Renal Allografts. J Histochem Cytochem. 2007 Jun;55(6):575-84.

Lee JH, Courtney M. Tate1, Jin-Sam You, and David G. Skalnik. 2007, Identification and Characterization of the Human Set1B Histone H3-Lys4 Methyltransferase Complex*. J. Biol. Chem., Vol. 282, Issue 18, 13419-13428, May 4, 2007

Luo Y, Kwon HJ, Montano S, Georgiadis M, Goebl MG, Harrington MA. Phosphorylation of SIMPL modulates RelA-associated NF-kappaB-dependent transcription. Am J Physiol Cell Physiol. 2007 Mar;292(3):C1013-23.

Mansson, L.E., K. Melican, J. Boekel, R.M. Sandoval, I. Hautefort, G.A. Tanner, B.A. Molitoris, and A. Richter-Dahlfors. 2007. Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. Cell Microbiol. 9:413-24.

Mansson, LE, K Melican, BA Molitoris, and A Richter-Dahlfors. 2007, Progression of Bacterial infections studied in real-time ? novel perspectives provided by multiphoton microscopy. Cell Microbiol. 9:2334-2343.

Mellon MJ, Ahn M, Jiménez JA, Kao C, Gardner TA. Anti-Angiogenic Gene Therapy for Metastatic Renal Cell Carcinoma Produces Tumor Growth Suppression in an Athymic Nude Mouse Model. J Urol. 2007 Dec 12.

Ming, W., S. Li, et al. (2007). "The Rac Effector p67phox Regulates Phagocyte NADPH Oxidase by Stimulating Vav1 Guanine Nucleotide Exchange Activity." Mol. Cell. Biol. 27(1): 312-323.

Molitoris B, Sandoval R, Ronco C, Bellomo R, Kellum JA (eds): Quantifying Dynamic Kidney Processes Utilizing Multi-Photon Microscopy; Acute Kidney Injury. Contrib Nephrol. Basel, Karger, 2007, vol 156, pp 227-235

Nakano Y, Banfi B, Jesaitis AJ, Dinauer MC, Allen LA, Nauseef WM. Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Biochem J. 2007 Apr 1;403(1):97-108.

Rondanino C, Rojas R, Ruiz WG, Wang E, Hughey RP, Dunn KW, Apodaca G. RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells. Traffic. 2007 Jul;8(7):932-49.

Rubart M, Field LJ. Cardiac Repair by ES-derived cells. In: Wobus A, Boheler K, eds. Handbook of Experimental Pharmacology: Stem Cells. Heidelberg, Germany: Springer-Verlag Publishers. (in press)

Russo LM, Sandoval RM, Brown D, Molitoris BA, Comper WD. Controversies in nephrology: response to 'renal albumin handling, facts, and artifacts'. Kidney Int. 2007 Nov;72(10):1195-7.

Russo LM, Sandoval RM, McKee M, Osicka TM, Collins AB, Brown D, Molitoris BA, Comper WD. The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states. Kidney Int. 2007 Mar;71(6):504-13.

Sandoval, R., and B.A. Molitoris. 2007. Quantifying Endocytosis in vivo Using Intravital Two-Photon Microscopy. Methods in Molecular Cell Biology. in press.

Sandoval RM, Reilly JP, Running W, Campos SB, Santos JR, Phillips CL, Molitoris BA. A non-nephrotoxic gentamicin congener that retains antimicrobial efficacy. J Am Soc Nephrol. 2006 Oct;17(10):2697-705.

Satpathy M, Cao L, Pincheira R, Emerson R, Bigsby R, Nakshatri H, Matei D. Enhanced peritoneal ovarian tumor dissemination by tissue transglutaminase. Cancer Res. 2007 Aug 1;67(15):7194-202.

Tao W, Evans BG, Yao J, Cooper S, Cornetta K, Ballas CB, Hangoc G, Broxmeyer HE. Enhanced green fluorescent protein is a nearly ideal long-term expression tracer for hematopoietic stem cells, whereas DsRed-express fluorescent protein is not. Stem Cells. 2007 Mar;25(3):670-8.

Temm C, Dominguez JH. Microcirculation: nexus of comorbidities in diabetes. Am J Physiol Renal Physiol. 2007 Aug;293(2):F486-93.

Wilson AL, Shen YC, Babb-Clendenon SG, Rostedt J, Liu B, Barald KF, Marrs JA, Liu Q. Cadherin-4 plays a role in the development of zebrafish cranial ganglia and lateral line system. Dev Dyn. 2007 Mar;236(3):893-902.

Xu C, Rossetti S, Jiang L, Harris PC, Brown-Glaberman U, Wandinger-Ness A, Bacallao R, Alper SL. Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling. Am J Physiol Renal Physiol. 2007 Mar;292(3):F930-45.

Xu J, Peng H, Chen Q, Liu Y, Dong Z, Zhang JT. Oligomerization domain of the multidrug resistance-associated transporter ABCG2 and its dominant inhibitory activity. Cancer Res. 2007 May 1;67(9):4373-81

Yoder MC, Mead LE, Prater D, Krier TR, Mroueh KN, Li F, Krasich R, Temm CJ, Prchal JT, Ingram DA. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals. Blood. 2007 Mar 1;109(5):1801-9.

Yu W, Sandoval RM, Molitoris BA. Rapid determination of renal filtration function using an optical ratiometric imaging approach. Am J Physiol Renal Physiol. 2007 Jun;292(6):F1873-80.

Zhou, F.C., B. Anthony, K.W. Dunn, W.B. Lindquist, Z.C. Xu, and P. Deng. 2007. Chronic alcohol drinking alters neuronal dendritic spines in the brain reward center nucleus accumbens. Brain Res. 1134:148-61.

Wagner, M.C., E.E. Molnar, B.A. Molitoris, and M.G. Goebl. 2006. Loss of the homotypic fusion and vacuole protein sorting or golgi-associated retrograde protein vesicle tethering complexes results in gentamicin sensitivity in the yeast Saccharomyces cerevisiae. Antimicrob Agents Chemother. 50:587-95.

Suurna, M.V., S.L. Ashworth, M. Hosford, R.M. Sandoval, S.E. Wean, B.M. Shah, J.R. Bamburg, and B.A. Molitoris. 2006. Cofilin mediates ATP depletion-induced endothelial cell actin alterations. Am J Physiol Renal Physiol. 290:F1398-407.

Sutton, T.A., M. Horbelt, and R.M. Sandoval. 2006. Imaging vascular pathology. Nephron Physiol. 103:p82-5.<\p>

Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC. The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis. J Exp Med. 2006 Aug 7;203(8):1915-25.

Sandoval, R.M., J.P. Reilly, W. Running, S.B. Campos, J.R. Santos, C.L. Phillips, and B.A. Molitoris. 2006. A non-nephrotoxic gentamicin congener that retains antimicrobial efficacy. J Am Soc Nephrol. 17:2697-705.

Rubart, M., and L.J. Field. 2006. Cardiac repair by embryonic stem-derived cells. Handb Exp Pharmacol:73-100.

Phillips, C.L., V.H. Gattone, 2nd, and S.M. Bonsib. 2006. Imaging glomeruli in renal biopsy specimens. Nephron Physiol. 103:p75-81.

Molitoris, B.A., and R.M. Sandoval. 2006. Pharmacophotonics: utilizing multi-photon microscopy to quantify drug delivery and intracellular trafficking in the kidney. Adv Drug Deliv Rev. 58:809-23.

Herget-Rosenthal, S., and B.A. Molitoris. 2006. Advances in imaging the kidney and cellular processes. Nephron Exp Nephrol. 103:e31-32.

Gopalakrishnan, S., M.A. Hallett, S. Atkinson, and J.A. Marrs. 2006. aPKC-Par complex dysfunction and tight junction disassembly in renal epithelial cells during ATP-depletion. Am J Physiol Cell Physiol.

El-Achkar, T.M., X. Huang, Z. Plotkin, R.M. Sandoval, G.J. Rhodes, and P.C. Dagher. 2006. Sepsis induces changes in the expression and distribution of Toll-like receptor 4 in the rat kidney. Am J Physiol Renal Physiol. 290:F1034-43.

Dunn, K.W., and P.A. Young. 2006. Principles of multiphoton microscopy. Nephron Exp Nephrol. 103:e33-40.

Clendenon, J.L., J.M. Byars, and D.P. Hyink. 2006. Image processing software for 3D light microscopy. Nephron Exp Nephrol. 103:e50-4.

Bauer, M.E., C.A. Townsend, A.R. Ronald, and S.M. Spinola. 2006. Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Microbes Infect. 8:2465-8.

Babbey, C.M., N. Ahktar, E. Wang, C.C. Chen, B.D. Grant, and K.W. Dunn. 2006. Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells. Mol Biol Cell. 17:3156-75.

Babb-Clendenon, S., Y.C. Shen, Q. Liu, K.E. Turner, M.S. Mills, G.W. Cook, C.A. Miller, V.H. Gattone, 2nd, K.F. Barald, and J.A. Marrs. 2006. Cadherin-2 participates in the morphogenesis of the zebrafish inner ear. J Cell Sci. 119:5169-77.

Atkinson, S.J. 2006. Functional intravital imaging of leukocytes in animal models of renal injury. Nephron Physiol. 103:p86-90.

Ashworth, S.L., and G.A. Tanner. 2006. Fluorescent labeling of renal cells in vivo. Nephron Physiol. 103:p91-6.

Yu, W., R.M. Sandoval, and B.A. Molitoris. 2005. Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies. Am J Physiol Cell Physiol. 289:C1197-208.

Yamauchi, A., C.C. Marchal, J. Molitoris, N. Pech, U. Knaus, J. Towe, S.J. Atkinson, and M.C. Dinauer. 2005. Rac GTPase isoform-specific regulation of NADPH oxidase and chemotaxis in murine neutrophils in vivo. Role of the C-terminal polybasic domain. J Biol Chem. 280:953-64.

Wang, E., C.M. Babbey, and K.W. Dunn. 2005. Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems. J Microsc. 218:148-59.

Tanner, G.A., R.M. Sandoval, B.A. Molitoris, J.R. Bamburg, and S.L. Ashworth. 2005. Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. Am J Physiol Renal Physiol. 289:F638-43.

Sutton, T.A., K.J. Kelly, H.E. Mang, Z. Plotkin, R.M. Sandoval, and P.C. Dagher. 2005. Minocycline reduces renal microvascular leakage in a rat model of ischemic renal injury. Am J Physiol Renal Physiol. 288:F91-7.

Raikwar, S.P., C.J. Temm, N.S. Raikwar, C. Kao, B.A. Molitoris, and T.A. Gardner. 2005. Adenoviral vectors expressing human endostatin-angiostatin and soluble Tie2: enhanced suppression of tumor growth and antiangiogenic effects in a prostate tumor model. Mol Ther. 12:1091-100.

Molitoris, B.A., and R.M. Sandoval. 2005. Intravital multiphoton microscopy of dynamic renal processes. Am J Physiol Renal Physiol. 288:F1084-9.

Molitoris, B.A. 2005. Microscopy as a quantitative science. Int J Artif Organs. 28:663-5.

Misseri, R., D.R. Meldrum, C.A. Dinarello, P. Dagher, K.L. Hile, R.C. Rink, and K.K. Meldrum. 2005. TNF-alpha mediates obstruction-induced renal tubular cell apoptosis and proapoptotic signaling. Am J Physiol Renal Physiol. 288:F406-11.

Li, W., M.J. Ferkowicz, S.A. Johnson, W.C. Shelley, and M.C. Yoder. 2005. Endothelial cells in the early murine yolk sac give rise to CD41-expressing hematopoietic cells. Stem Cells Dev. 14:44-54.

Lahvis, G.P., R.W. Pyzalski, E. Glover, H.C. Pitot, M.K. McElwee, and C.A. Bradfield. 2005. The aryl hydrocarbon receptor is required for developmental closure of the ductus venosus in the neonatal mouse. Mol Pharmacol. 67:714-20.

Komlosi, P., A. Fintha, and P.D. Bell. 2005. Renal cell-to-cell communication via extracellular ATP. Physiology (Bethesda). 20:86-90.

Goodfellow, J.A., T. Bowes, K. Sheikh, M. Odaka, S.K. Halstead, P.D. Humphreys, E.R. Wagner, N. Yuki, K. Furukawa, K. Furukawa, J.J. Plomp, and H.J. Willison. 2005. Overexpression of GD1a ganglioside sensitizes motor nerve terminals to anti-GD1a antibody-mediated injury in a model of acute motor axonal neuropathy. J Neurosci. 25:1620-8.

Ferkowicz, M.J., and M.C. Yoder. 2005. Blood island formation: longstanding observations and modern interpretations. Exp Hematol. 33:1041-1047.

Fehrenbacher, J.C., T.H. Burkey, G.D. Nicol, and M.R. Vasko. 2005. Tumor necrosis factor alpha and interleukin-1beta stimulate the expression of cyclooxygenase II but do not alter prostaglandin E2 receptor mRNA levels in cultured dorsal root ganglia cells. Pain. 113:113-22.

Babb, S.G., S.M. Kotradi, B. Shah, C. Chiappini-Williamson, L.N. Bell, G. Schmeiser, E. Chen, Q. Liu, and J.A. Marrs. 2005. Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Dev Dyn. 233:930-45.

Zhang, D., J. Sherwood, L. Li, and D.R. Swartz. 2004. Unloaded shortening velocity in single permeabilized vascular smooth muscle cells is independent of microtubule status. J Muscle Res Cell Motil. 25:167-75.

Yamauchi, A., C. Kim, S. Li, C.C. Marchal, J. Towe, S.J. Atkinson, and M.C. Dinauer. 2004. Rac2-deficient murine macrophages have selective defects in superoxide production and phagocytosis of opsonized particles. J Immunol. 173:5971-9.

Tanner, G.A., R.M. Sandoval, and K.W. Dunn. 2004. Two-photon in vivo microscopy of sulfonefluorescein secretion in normal and cystic rat kidneys. Am J Physiol Renal Physiol. 286:F152-60.

Sandoval, R.M., and B.A. Molitoris. 2004. Gentamicin traffics retrograde through the secretory pathway and is released in the cytosol via the endoplasmic reticulum. Am J Physiol Renal Physiol. 286:F617-24.

Sandoval, R.M., M.D. Kennedy, P.S. Low, and B.A. Molitoris. 2004. Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy. Am J Physiol Cell Physiol. 287:C517-26.

Rubart, M., M.H. Soonpaa, H. Nakajima, and L.J. Field. 2004. Spontaneous and evoked intracellular calcium transients in donor-derived myocytes following intracardiac myoblast transplantation. J Clin Invest. 114:775-83.

Rubart, M. 2004. Two-photon microscopy of cells and tissue. Circ Res. 95:1154-66.

Rehman, J., J. Li, L. Parvathaneni, G. Karlsson, V.R. Panchal, C.J. Temm, J. Mahenthiran, and K.L. March. 2004. Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells. J Am Coll Cardiol. 43:2314-8.

Phillips, C.L., K.J. Miller, A.J. Filson, J. Nurnberger, J.L. Clendenon, G.W. Cook, K.W. Dunn, P.A. Overbeek, V.H. Gattone, 2nd, and R.L. Bacallao. 2004. Renal cysts of inv/inv mice resemble early infantile nephronophthisis. J Am Soc Nephrol. 15:1744-55.

Nurnberger, J., A. Kribben, A. Opazo Saez, G. Heusch, T. Philipp, and C.L. Phillips. 2004. The Invs gene encodes a microtubule-associated protein. J Am Soc Nephrol. 15:1700-10. Molitoris, B.A., and T.A. Sutton. 2004. Endothelial injury and dysfunction: role in the extension phase of acute renal failure. Kidney Int. 66:496-9.

Molitoris, B.A. 2004. Actin cytoskeleton in ischemic acute renal failure. Kidney Int. 66:871-83.

Larson, D.M., M.A. Kennedy, R.F. Bowen, C.B. Verchere, and M.A. Deeg. 2004. Glycosylphosphatidylinositol-specific phospholipase D immunoreactivity is present in islet amyloid in type 2 diabetes. J Pathol. 203:961-7.

Koura, Y., A. Ichihara, Y. Tada, Y. Kaneshiro, H. Okada, C.J. Temm, M. Hayashi, and T. Saruta. 2004. Anandamide decreases glomerular filtration rate through predominant vasodilation of efferent arterioles in rat kidneys. J Am Soc Nephrol. 15:1488-94.

Kelly, K.J., T.A. Sutton, N. Weathered, N. Ray, E.J. Caldwell, Z. Plotkin, and P.C. Dagher. 2004. Minocycline inhibits apoptosis and inflammation in a rat model of ischemic renal injury. Am J Physiol Renal Physiol. 287:F760-6.

Guy, J.C., C.S. Hunter, A.D. Showalter, T.P. Smith, K. Charoonpatrapong, K.W. Sloop, J.P. Bidwell, and S.J. Rhodes. 2004. Conserved amino acid sequences confer nuclear localization upon the Prophet of Pit-1 pituitary transcription factor protein. Gene. 336:263-73.

Gustin, J.A., R. Pincheira, L.D. Mayo, O.N. Ozes, K.M. Kessler, M.R. Baerwald, C.K. Korgaonkar, and D.B. Donner. 2004. Tumor necrosis factor activates CRE-binding protein through a p38 MAPK/MSK1 signaling pathway in endothelial cells. Am J Physiol Cell Physiol. 286:C547-55.

Gattone, V.H., 2nd, B.A. Tourkow, C.M. Trambaugh, A.C. Yu, S. Whelan, C.L. Phillips, P.C. Harris, and R.G. Peterson. 2004. Development of multiorgan pathology in the wpk rat model of polycystic kidney disease. Anat Rec A Discov Mol Cell Evol Biol. 277:384-95.

Blazer-Yost, B.L., J.C. Vahle, J.M. Byars, and R.L. Bacallao. 2004. Real-time three-dimensional imaging of lipid signal transduction: apical membrane insertion of epithelial Na(+) channels. Am J Physiol Cell Physiol. 287:C1569-76.

Bishop, D.L., T. Misgeld, M.K. Walsh, W.B. Gan, and J.W. Lichtman. 2004. Axon branch removal at developing synapses by axosome shedding. Neuron. 44:651-61.

Babb, S.G., and J.A. Marrs. 2004. E-cadherin regulates cell movements and tissue formation in early zebrafish embryos. Dev Dyn. 230:263-77.

Ashworth, S.L., S.E. Wean, S.B. Campos, C.J. Temm-Grove, E.L. Southgate, B. Vrhovski, P. Gunning, R.P. Weinberger, and B.A. Molitoris. 2004. Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments. Am J Physiol Renal Physiol. 286:F988-96.

Stanley M. Spinola, Cliffton T. H. Bong, Andrew L. Faber, Kate R. Fortney, Stacy L. Bennett, Carisa A. Townsend, Beth E. Zwickl, Steven D. Billings,Tricia L. Humphreys, Margaret E. Bauer,and Barry P. Katz. Differences in Host Susceptibility to Disease Progression in the Human Challenge Model of Haemophilus ducreyi Infection. INFECTION AND IMMUNITY, Nov. 2003, p. 6658?6663

Yang, F.C., D.A. Ingram, S. Chen, C.M. Hingtgen, N. Ratner, K.R. Monk, T. Clegg, H. White, L. Mead, M.J. Wenning, D.A. Williams, R. Kapur, S.J. Atkinson, D.W. Clapp, and A. Ichihara. 2003. Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for Nf1+/- mast cells. J Clin Invest. 112:1851-61.

Ward, C.J., D. Yuan, T.V. Masyuk, X. Wang, R. Punyashthiti, S. Whelan, R. Bacallao, R. Torra, N.F. LaRusso, V.E. Torres, and P.C. Harris. 2003. Cellular and subcellular localization of the ARPKD protein; fibrocystin is expressed on primary cilia. Hum Mol Genet. 12:2703-10.

Sutton, T.A., H.E. Mang, S.B. Campos, R.M. Sandoval, M.C. Yoder, and B.A. Molitoris. 2003. Injury of the renal microvascular endothelium alters barrier function after ischemia. Am J Physiol Renal Physiol. 285:F191-8.

Stanic, A.K., A.D. De Silva, J.J. Park, V. Sriram, S. Ichikawa, Y. Hirabyashi, K. Hayakawa, L. Van Kaer, R.R. Brutkiewicz, and S. Joyce. 2003. Defective presentation of the CD1d1-restricted natural Va14Ja18 NKT lymphocyte antigen caused by beta-D-glucosylceramide synthase deficiency. Proc Natl Acad Sci U S A. 100:1849-54.

Rubart, M., E. Wang, K.W. Dunn, and L.J. Field. 2003. Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts. Am J Physiol Cell Physiol. 284:C1654-68.

Rubart, M., K.B. Pasumarthi, H. Nakajima, M.H. Soonpaa, H.O. Nakajima, and L.J. Field. 2003. Physiological coupling of donor and host cardiomyocytes after cellular transplantation. Circ Res. 92:1217-24.

Magy, N., J.J. Liepnieks, M.D. Benson, and B. Kluve-Beckerman. 2003. Amyloid-enhancing factor mediates amyloid formation on fibroblasts via a nidus/template mechanism. Arthritis Rheum. 48:1430-7.

Kelly, K.J., R.M. Sandoval, K.W. Dunn, B.A. Molitoris, and P.C. Dagher. 2003. A novel method to determine specificity and sensitivity of the TUNEL reaction in the quantitation of apoptosis. Am J Physiol Cell Physiol. 284:C1309-18.

Kelly, K.J., Z. Plotkin, S.L. Vulgamott, and P.C. Dagher. 2003. P53 mediates the apoptotic response to GTP depletion after renal ischemia-reperfusion: protective role of a p53 inhibitor. J Am Soc Nephrol. 14:128-38.

Hallett, M.A., P.C. Dagher, and S.J. Atkinson. 2003. Rho GTPases show differential sensitivity to nucleotide triphosphate depletion in a model of ischemic cell injury. Am J Physiol Cell Physiol. 285:C129-38.

Gopalakrishnan, S., M.A. Hallett, S.J. Atkinson, and J.A. Marrs. 2003. Differential regulation of junctional complex assembly in renal epithelial cell lines. Am J Physiol Cell Physiol. 285:C102-11.

Gopalakrishnan, S., K.W. Dunn, and J.A. Marrs. 2003. Testing effects of signal transduction pathways on cadherin junctional complex assembly using quantitative image analysis. Methods. 30:218-27.

Ferkowicz, M.J., M. Starr, X. Xie, W. Li, S.A. Johnson, W.C. Shelley, P.R. Morrison, and M.C. Yoder. 2003. CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 130:4393-4403.

Dunn, K.W., R.M. Sandoval, and B.A. Molitoris. 2003. Intravital imaging of the kidney using multiparameter multiphoton microscopy. Nephron Exp Nephrol. 94:e7-11.

Dowell, J.D., M. Rubart, K.B. Pasumarthi, M.H. Soonpaa, and L.J. Field. 2003. Myocyte and myogenic stem cell transplantation in the heart. Cardiovasc Res. 58:336-50.

Dagher, P.C., S. Herget-Rosenthal, S.G. Ruehm, S.K. Jo, R.A. Star, R. Agarwal, and B.A. Molitoris. 2003. Newly developed techniques to study and diagnose acute renal failure. J Am Soc Nephrol. 14:2188-98.

Bacallao, R.L., W. Yu, K.W. Dunn, and C.L. Phillips. 2003. Novel light microscopy imaging techniques in nephrology. Curr Opin Nephrol Hypertens. 12:455-61.

Ashworth, S.L., E.L. Southgate, R.M. Sandoval, P.J. Meberg, J.R. Bamburg, and B.A. Molitoris. 2003. ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion. Am J Physiol Renal Physiol. 284:F852-62.

Tao, W., M.D. Filippi, J.R. Bailey, S.J. Atkinson, B. Connors, A. Evan, and D.A. Williams. 2002. The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization. Blood. 100:1679-88.

Sutton, T.A., C.J. Fisher, and B.A. Molitoris. 2002. Microvascular endothelial injury and dysfunction during ischemic acute renal failure. Kidney Int. 62:1539-49.

Spinola, S.M., M.E. Bauer, and R.S. Munson, Jr. 2002. Immunopathogenesis of Haemophilus ducreyi infection (chancroid). Infect Immun. 70:1667-76.

Seo, Y.R., M.L. Fishel, S. Amundson, M.R. Kelley, and M.L. Smith. 2002. Implication of p53 in base excision DNA repair: in vivo evidence. Oncogene. 21:731-7.

Sandoval, R.M., R.L. Bacallao, K.W. Dunn, J.D. Leiser, and B.A. Molitoris. 2002. Nucleotide depletion increases trafficking of gentamicin to the Golgi complex in LLC-PK1 cells. Am J Physiol Renal Physiol. 283:F1422-9.

Roberts, T.J., V. Sriram, P.M. Spence, M. Gui, K. Hayakawa, I. Bacik, J.R. Bennink, J.W. Yewdell, and R.R. Brutkiewicz. 2002. Recycling CD1d1 molecules present endogenous antigens processed in an endocytic compartment to NKT cells. J Immunol. 168:5409-14.

Nurnberger, J., R.L. Bacallao, and C.L. Phillips. 2002. Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells. Mol Biol Cell. 13:3096-106.

Molitoris, B.A., R. Sandoval, and T.A. Sutton. 2002. Endothelial injury and dysfunction in ischemic acute renal failure. Crit Care Med. 30:S235-40.

Malone, J.C., A.F. Hood, T. Conley, J. Nurnberger, L.A. Baldridge, J.L. Clendenon, K.W. Dunn, and C.L. Phillips. 2002. Three-dimensional imaging of human skin and mucosa by two-photon laser scanning microscopy. J Cutan Pathol. 29:453-8.

Kwon, O., C.L. Phillips, and B.A. Molitoris. 2002. Ischemia induces alterations in actin filaments in renal vascular smooth muscle cells. Am J Physiol Renal Physiol. 282:F1012-9.

Kluve-Beckerman, B., J.J. Manaloor, and J.J. Liepnieks. 2002. A pulse-chase study tracking the conversion of macrophage-endocytosed serum amyloid A into extracellular amyloid. Arthritis Rheum. 46:1905-13.

Gopalakrishnan, S., K.W. Dunn, and J.A. Marrs. 2002. Rac1, but not RhoA, signaling protects epithelial adherens junction assembly during ATP depletion. Am J Physiol Cell Physiol. 283:C261-72.

Girton, R.A., D.P. Sundin, and M.E. Rosenberg. 2002. Clusterin protects renal tubular epithelial cells from gentamicin-mediated cytotoxicity. Am J Physiol Renal Physiol. 282:F703-9.

Dunn, K.W., R.M. Sandoval, K.J. Kelly, P.C. Dagher, G.A. Tanner, S.J. Atkinson, R.L. Bacallao, and B.A. Molitoris. 2002. Functional studies of the kidney of living animals using multicolor two-photon microscopy. Am J Physiol Cell Physiol. 283:C905-16.

Clendenon, J.L., C.L. Phillips, R.M. Sandoval, S. Fang, and K.W. Dunn. 2002. Voxx: a PC-based, near real-time volume rendering system for biological microscopy. Am J Physiol Cell Physiol. 282:C213-8.

Batourina, E., C. Choi, N. Paragas, N. Bello, T. Hensle, F.D. Costantini, A. Schuchardt, R.L. Bacallao, and C.L. Mendelsohn. 2002. Distal ureter morphogenesis depends on epithelial cell remodeling mediated by vitamin A and Ret. Nat Genet. 32:109-15.

Zhu, W.G., J.D. Seno, B.D. Beck, and J.R. Dynlacht. 2001. Translocation of MRE11 from the nucleus to the cytoplasm as a mechanism of radiosensitization by heat. Radiat Res. 156:95-102.

Wang, E., J.G. Pennington, J.R. Goldenring, W. Hunziker, and K.W. Dunn. 2001. Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells. J Cell Sci. 114:3309-21.

Sutton, T.A., H.E. Mang, and S.J. Atkinson. 2001. Rho-kinase regulates myosin II activation in MDCK cells during recovery after ATP depletion. Am J Physiol Renal Physiol. 281:F810-8.

Sundin, D.P., R. Sandoval, and B.A. Molitoris. 2001. Gentamicin inhibits renal protein and phospholipid metabolism in rats: implications involving intracellular trafficking. J Am Soc Nephrol. 12:114-23.

Mayo, L.D., K.M. Kessler, R. Pincheira, R.S. Warren, and D.B. Donner. 2001. Vascular endothelial cell growth factor activates CRE-binding protein by signaling through the KDR receptor tyrosine kinase. J Biol Chem. 276:25184-9.

Knepler, J.L., Jr., L.N. Taher, M.P. Gupta, C. Patterson, F. Pavalko, M.D. Ober, and C.M. Hart. 2001. Peroxynitrite causes endothelial cell monolayer barrier dysfunction. Am J Physiol Cell Physiol. 281:C1064-75.

Kluve-Beckerman, B., J. Manaloor, and J.J. Liepnieks. 2001. Binding, trafficking and accumulation of serum amyloid A in peritoneal macrophages. Scand J Immunol. 53:393-400.

Kher, R., and R. Bacallao. 2001. Direct in situ reverse transcriptase-polymerase chain reaction. Am J Physiol Cell Physiol. 281:C726-32.

Kelly, K.J., Z. Plotkin, and P.C. Dagher. 2001. Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury. J Clin Invest. 108:1291-8.

Kelly, K.J., N.R. Baird, and A.L. Greene. 2001. Induction of stress response proteins and experimental renal ischemia/reperfusion. Kidney Int. 59:1798-802.

Herget-Rosenthal, S., M. Hosford, A. Kribben, S.J. Atkinson, R.M. Sandoval, and B.A. Molitoris. 2001. Characteristics of EYFP-actin and visualization of actin dynamics during ATP depletion and repletion. Am J Physiol Cell Physiol. 281:C1858-70.

Beck, B.D., and J.R. Dynlacht. 2001. Heat-induced aggregation of XRCC5 (Ku80) in nontolerant and thermotolerant cells. Radiat Res. 156:767-74.

Bauer, M.E., M.P. Goheen, C.A. Townsend, and S.M. Spinola. 2001. Haemophilus ducreyi associates with phagocytes, collagen, and fibrin and remains extracellular throughout infection of human volunteers. Infect Immun. 69:2549-57.

Batiuk, T.D., C. Schnizlein-Bick, Z. Plotkin, and P.C. Dagher. 2001. Guanine nucleosides and Jurkat cell death: roles of ATP depletion and accumulation of deoxyribonucleotides. Am J Physiol Cell Physiol. 281:C1776-84.

Babb, S.G., J. Barnett, A.L. Doedens, N. Cobb, Q. Liu, B.C. Sorkin, P.C. Yelick, P.A. Raymond, and J.A. Marrs. 2001. Zebrafish E-cadherin: expression during early embryogenesis and regulation during brain development. Dev Dyn. 221:231-7.

Ashworth, S.L., R.M. Sandoval, M. Hosford, J.R. Bamburg, and B.A. Molitoris. 2001. Ischemic injury induces ADF relocalization to the apical domain of rat proximal tubule cells. Am J Physiol Renal Physiol. 280:F886-94.

Wang, E., M.D. Lee, and K.W. Dunn. 2000. Lysosomal accumulation of drugs in drug-sensitive MES-SA but not multidrug-resistant MES-SA/Dx5 uterine sarcoma cells. J Cell Physiol. 184:263-74.

Wang, E., P.S. Brown, B. Aroeti, S.J. Chapin, K.E. Mostov, and K.W. Dunn. 2000. Apical and basolateral endocytic pathways of MDCK cells meet in acidic common endosomes distinct from a nearly-neutral apical recycling endosome. Traffic. 1:480-93.

Troxell, M.L., S. Gopalakrishnan, J. McCormack, B.A. Poteat, J. Pennington, S.M. Garringer, E.E. Schneeberger, W.J. Nelson, and J.A. Marrs. 2000. Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. J Cell Sci. 113 ( Pt 6):985-96.

Sandoval, R.M., K.W. Dunn, and B.A. Molitoris. 2000. Gentamicin traffics rapidly and directly to the Golgi complex in LLC-PK(1) cells. Am J Physiol Renal Physiol. 279:F884-90.

Fortney, K.R., R.S. Young, M.E. Bauer, B.P. Katz, A.F. Hood, R.S. Munson, Jr., and S.M. Spinola. 2000. Expression of peptidoglycan-associated lipoprotein is required for virulence in the human model of Haemophilus ducreyi infection. Infect Immun. 68:6441-8.

Fang, S., Y. Dai, F. Myers, M. Tuceryan, and K. Dunn. 2000. Three-dimensional microscopy data exploration by interactive volume visualization. Scanning. 22:218-26.

Dunn, K.W., and E. Wang. 2000. Optical aberrations and objective choice in multicolor confocal microscopy. Biotechniques. 28:542-4, 546, 548-50.

Dagher, P.C. 2000. Modeling ischemia in vitro: selective depletion of adenine and guanine nucleotide pools. Am J Physiol Cell Physiol. 279:C1270-7.

Brown, P.S., E. Wang, B. Aroeti, S.J. Chapin, K.E. Mostov, and K.W. Dunn. 2000. Definition of distinct compartments in polarized Madin-Darby canine kidney (MDCK) cells for membrane-volume sorting, polarized sorting and apical recycling. Traffic. 1:124-40.

Bauer, M.E., and S.M. Spinola. 2000. Localization of Haemophilus ducreyi at the pustular stage of disease in the human model of infection. Infect Immun. 68:2309-14.

Al-Tawfiq, J.A., M.E. Bauer, K.R. Fortney, B.P. Katz, A.F. Hood, M. Ketterer, M.A. Apicella, and S.M. Spinola. 2000. A pilus-deficient mutant of Haemophilus ducreyi is virulent in the human model of experimental infection. J Infect Dis. 181:1176-9.

Zong, H., N. Raman, L.A. Mickelson-Young, S.J. Atkinson, and L.A. Quilliam. 1999. Loop 6 of RhoA confers specificity for effector binding, stress fiber formation, and cellular transformation. J Biol Chem. 274:4551-60.

Troxell, M.L., Y.T. Chen, N. Cobb, W.J. Nelson, and J.A. Marrs. 1999. Cadherin function in junctional complex rearrangement and posttranslational control of cadherin expression. Am J Physiol. 276:C404-18.

Schwartz, N., M. Hosford, R.M. Sandoval, M.C. Wagner, S.J. Atkinson, J. Bamburg, and B.A. Molitoris. 1999. Ischemia activates actin depolymerizing factor: role in proximal tubule microvillar actin alterations. Am J Physiol. 276:F544-51.

Raman, N., and S.J. Atkinson. 1999. Rho controls actin cytoskeletal assembly in renal epithelial cells during ATP depletion and recovery. Am J Physiol. 276:C1312-24.

Mehta, D., and S.J. Gunst. 1999. Actin polymerization stimulated by contractile activation regulates force development in canine tracheal smooth muscle. J Physiol. 519 Pt 3:829-40.

Ashworth, S.L., and B.A. Molitoris. 1999. Pathophysiology and functional significance of apical membrane disruption during ischemia. Curr Opin Nephrol Hypertens. 8:449-58.

Yu, L., M.T. Quinn, A.R. Cross, and M.C. Dinauer. 1998. Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase. Proc Natl Acad Sci U S A. 95:7993-8.

Sutton, T.A., and B.A. Molitoris. 1998. Mechanisms of cellular injury in ischemic acute renal failure. Semin Nephrol. 18:490-7.

Sandoval, R., J. Leiser, and B.A. Molitoris. 1998. Aminoglycoside antibiotics traffic to the Golgi complex in LLC-PK1 cells. J Am Soc Nephrol. 9:167-74.

Gopalakrishnan, S., N. Raman, S.J. Atkinson, and J.A. Marrs. 1998. Rho GTPase signaling regulates tight junction assembly and protects tight junctions during ATP depletion. Am J Physiol. 275:C798-809.

Dunn, K., and F.R. Maxfield. 1998. Ratio imaging instrumentation. Methods Cell Biol. 56:217-36.

Dunn, K., and R. Bacallao. 1998. Optical methods in renal research. Semin Nephrol. 18:122-37.

1997

Wagner, M.C., and B.A. Molitoris. 1997. ATP depletion alters myosin I beta cellular location in LLC-PK1 cells. Am J Physiol. 272:C1680-90.

Sundin, D.P., C. Meyer, R. Dahl, A. Geerdes, R. Sandoval, and B.A. Molitoris. 1997. Cellular mechanism of aminoglycoside tolerance in long-term gentamicin treatment. Am J Physiol. 272:C1309-18.

Deeg, M.A., and C.B. Verchere. 1997. Regulation of glycosylphosphatidylinositol-specific phospholipase D secretion from beta TC3 cells. Endocrinology. 138:819-26.

Molitoris, B.A., R. Dahl, and M. Hosford. 1996. Cellular ATP depletion induces disruption of the spectrin cytoskeletal network. Am J Physiol. 271:F790-8.