Indiana Center for Biological Microscopy

• About
  • Overview
  • Faculty/Staff
• Using the facility
  • Equipment
  • Usage Policy
• Tutorials
  • Colocalization
  • FRET
  • Live Cell
  • Other Protocols
• Research
  • Obrien Center
    • Funding Opportunities
    • Workshops
  • Links
  • Movies
    • Movies of the kidney
    • Movies of cells
    • Other Voxx movies
    • Segmentation movies
    • Anaglyph stereo movies
    • Intravital movies of the kidney
  • Publications
• Software
• Scheduling

Protocols, Application Notes and FAQs

There are many established protocols for fluorescence staining, imaging and image processing. Your best guide is the literature within your field. We have placed some basic protocols here.

• Imaging Live Cells
  • Tips for Successful Live Cell Imaging
  • Glass Bottom Dishes
• Imaging of Fixed Cells
  • Getting started
  • Standard Immunofluorescence Protocol
  • Making 4% para-formaldehyde
• FRET
  • FRET Intro
  • Articles on FRET
• Image Processing and Analysis
  • Analysis of Colocalization using Metamorph
• User Guides
  • Biorad User Manual
  • Widefield with Orca Camera Manual