Intravital Microscopy of the Kidney

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Simon Macrophage
Transplanted GFP-labeled macrophage crawling on endothelia in kidney of living rat following renal ischemia.
Simon Atkinson

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Facility infusion
Endocytosis of TexasRed gentamicin by proximal gtubule cells of a living rat.
Ruben Sandoval and Bruce Molitoris

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Tm-rat1-06min
Blood flow and proximal tubule endocytosis in kidney of a living rat.
Ruben Sandoval and Timothy Sutton

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Obstruction
Passage of a tubular cast in kidney of a living rat.
Ruben Sandoval and Bruce Molitoris

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Casc infusion
Filtration and tubular concentration of a small molecular-weight dextran in the kidney of a living rat.
Ruben Sandoval and Bruce Molitoris

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Glom movie
Filtration and proximal tubule endocytosis of a small molecular weight dextran.
Ruben Sandoval and Bruce Molitoris

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Dunn movie5
Blood flow and proximal tubule endocytosis in the kidney of a living rat.
Ruben Sandoval and Bruce Molitoris

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Live rat tubules
3D reconstruction of a volume collected in the kidney of a living rat using 2 photon microscopy. The rat was injected with a 500Kd green dextran, Hoechst and Texas-Red gentamicin. The large dextran is retained in the capillaries, while the gentamicin is filtered into the renal tubules where it is endocytosed in proximal tubules and concentrated in the lumen of distal tubules.
Ruben Sandoval and Bruce Molitoris