Sorting in Polarized MDCK PTR Cells
Filter grown PTR cells were dual-labeled with IgA (green) and Tf (red). Colocalization of the two proteins is shown in the medial focal planes, but IgA is sorted from the common compartment to the apical compartment lacking Tf. (Dr. Dunn)

Endosomes and anti-cadherin
A two photon volume of texas-red labeled endosomes and fluorescein labeled anti-cadherin. (Dr. Dunn)

MCF-7 cell expressing GFP-Rab11
MCF-7 cell experssing GFP-Rab11 labeled with Alexa-568-Tf. (Dr. Dunn)

Two-photon volume of hippocampal neuron
Two-photon volume of hippocampal neuron.
(Zao Xu and Ken Dunn)

GFP-Rab7
MDCK PTR cells were transfected with GFP-Rab7. The movie acquired on the Applied Precision System.
(Dr. Dunn and Angela Wandinger-Ness, Univ. of New Mexico)

Nuclei and anti-cadherin
Two photon volume of dapi labeled nuclei and fluorescein labeled anti-cadherin. (Dr. Dunn)

Endosomes and anti-furin in the golgi
A two photon volume of texas-red labeled endosomes and fluorescein labeled anti-furin in the golgi. (Dr. Dunn)

Fluorescein labeled antibody to alpha tubulin
A deconvolved 3-D image (acquired with Delta Vision system, Applied Precision) has been rendered with VOXX program.
Tissue cultured PTR cells were labeled with TexasRed-Phalloidin, FITC-a-tubulin, and DAPI. (Drs. Wang and Dunn)

MDCK cells expressing GFP-tubulin
Voxx rendered 3d volume of living MDCK cells expressing GFP-tubulin collected with the Perkin-Elmer Ultraview system. (Dr. Dunn)

Living MDCK epithelial cell expressing GFP-tubulin
GFP-Rab7 labeled vesicles migrating on GFP-tubulin-labed microtubules in a living MDCK epithelial cell. (10X actual speed) Time series collected with the Perkin-Elmer Ultraview Microscope. (Dr. Dunn)

Mpeg4 movie may require software updates to view.

Living CHO cells expressing GFP-tubulin
GFP-Rab7 labeled vesicles migrating on GFP-tubulin-labed microtubules in a living CHO cell. (10X actual speed) Time series collected with the Perkin-Elmer Ultraview Microscope. (Dr. Dunn)

Mpeg4 movie may require software updates to view.

Living CHO fibroblast expressing GFP-tubulin
3D rendering of a living CHO fibroblast expressing GFP-tubulin. Volume rendered in real-time using Voxx. (Dr. Dunn)