Functional studies of the kidney of living animals using
multicolor 2-photon microscopy

Kenneth W. Dunn, Ruben M.Sandoval, Katherine J. Kelley, Pierre C. Dagher, George A. Tanner, Simon J. Atkinson,
Robert L. Bacallao, and Bruce A. Molitoris

dunn3.pdf - Journal article available in pdf format.

Movie for Figure 2A - Voxx-rendered image volume of the kidney of a living rat following tail-vein injection with Hoechst 33342 and propidium iodide.

Movie for Figure 2B - Voxx-rendered image volume of the kidney of a living rat injected with Hoechst 33342 and propidium iodide following brief renal ischemia.

Movie for Figure 3A - Movie loop of a 20 second time series collected from the kidney of a living rat that had been injected with Hoechst 33342, rhodamine-conjugated albumin and 10,000 molecular weight fluorescein-conjugated dextran.

Movie for Figure 3B - Movie loop of a 30 second time series collected from the kidney collected at the level of the glomerulus from a living rat that had been injected with Hoechst 33342 and a 500,000 molecular weight fluorescein conjugated dextran.

Movie for Figure 3F - Voxx-rendered image volume of the kidney of a living heterozygous Han-SPRD rat model of polycystic kidney disease following intravenous injection with Hoechst 33342 and 10,000 MW rhodamine dextran.

Movie for Figure 5C - Voxx-rendered image volume of the kidney of a living transgenic mouse expressing green fluorescent protein driven by an endothelial-specific promotor (Tie-2).

Movie for Figure 5D - Movie loop of a 6 second time series collected from the kidney of a mouse transplanted with bone marrow cells expressing enhanced green fluorescent protein.

Correspondence should be sent to:

Kenneth Dunn
Department of Medicine
Division of Nephrology
Indiana University Medical Center
1120 South Dr., FH 115 Indianapolis, IN 46202

Telephone: 317-278-0436
FAX: 317-274-8575
Email:kwdunn@iupui.edu